A high-sensitivity ELISA for detection of human FGF18 in culture supernatants from tumor cell lines

Yuichi Tsuchiya; Sachiko Komazawa-Sakon; Masato Tanaka; Takumi Kanokogi; Kenta Moriwaki; Hisaya Akiba; Hideo Yagita; Ko Okumura; Kevin C Entzminger; C J Okumura; Toshiaki Maruyama; Hiroyasu Nakano

doi:10.1016/j.bbrc.2023.06.070

A high-sensitivity ELISA for detection of human FGF18 in culture supernatants from tumor cell lines

Biochem Biophys Res Commun. 2023 Oct 1:675:71-77. doi: 10.1016/j.bbrc.2023.06.070. Epub 2023 Jul 7.

Authors

Affiliations

¹ Department of Biochemistry, Faculty of Medicine, Toho University, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540, Japan; Department of Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba 274-8510, Japan.
² Department of Biochemistry, Faculty of Medicine, Toho University, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540, Japan.
³ Laboratory of Immune regulation, School of Life Science, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji-shi, Tokyo, 192-0392, Japan.
⁴ Department of Biochemistry, Faculty of Medicine, Toho University, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540, Japan; Department of Respiratory Medicine, Omori Medical Center, Toho University School Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540, Japan.
⁵ Department of Immunology, Faculty of Medicine and Graduate School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
⁶ Atopy Research Center, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
⁷ Abwiz Bio, Inc., 9823 Pacific Heights BLVD, Suite J, San Diego, CA, 92121, USA.
⁸ Department of Biochemistry, Faculty of Medicine, Toho University, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540, Japan. Electronic address: hiroyasu.nakano@med.toho-u.ac.jp.

PMID: 37453260
DOI: 10.1016/j.bbrc.2023.06.070

Abstract

Fibroblast growth factor 18 (FGF18) is elevated in several human cancers, such as gastrointestinal and ovarian cancers, and stimulates the proliferation of tumor cells. This suggests that FGF18 may be a promising candidate biomarker in cancer patients. However, the lack of a high-sensitivity enzyme-linked immunosorbent assay (ELISA) does not permit testing of this possibility. In this study, we generated monoclonal antibodies against human FGF18 and developed a high-sensitivity ELISA to measure human FGF18 at concentrations as low as 10 pg/mL. Of the eight tumor cell lines investigated, we detected human FGF18 in culture supernatants from four tumor cell lines, including HeLa, OVCAR-3, BxPC-3, and SW620 cells, albeit the production levels were relatively low in the latter two cell lines. Moreover, the in-house ELISA could detect murine FGF18 in sera from mice overexpressing murine Fgf18 in hepatocytes, although the sensitivity in detecting murine FGF18 was relatively low. This FGF18 ELISA could be a valuable tool to validate FGF18 as a potential biomarker for cancer patients and to test the contribution of FGF18 for various disease models invivo and in vitro.

Keywords: Biomarker; Cancer; Enzyme-linked immunosorbent assay; Fibroblast growth factor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis*
Cell Line, Tumor
Enzyme-Linked Immunosorbent Assay
Female
Fibroblast Growth Factors / metabolism
Humans
Mice
Ovarian Neoplasms* / pathology

Substances

fibroblast growth factor 18
Fibroblast Growth Factors