Qualitative and Quantitative Analyses of the Enantiomers of Nicotine and Nornicotine Employing Chiral Supercritical Fluid Chromatography

Mehdi Ashraf-Khorassani; Thomas A Perfetti; Mike F Dube; William M Coleman; John M Ferraro; Weston J Umstead

doi:10.1093/chromsci/bmad048

Qualitative and Quantitative Analyses of the Enantiomers of Nicotine and Nornicotine Employing Chiral Supercritical Fluid Chromatography

J Chromatogr Sci. 2023 Jul 14:bmad048. doi: 10.1093/chromsci/bmad048. Online ahead of print.

Authors

Mehdi Ashraf-Khorassani¹, Thomas A Perfetti², Mike F Dube³, William M Coleman⁴, John M Ferraro⁵, Weston J Umstead⁵

Affiliations

¹ Department of Chemistry, Virginia Tech, 1040 Drillfiled Drive,171 Davidson Hall, Blacksburg, VA 24061, USA.
² Perfetti and Perfetti, LLC, Winston-Salem, NC 27103, USA.
³ Dube180Consulting, LLC, Winston-Salem, NC 27106, USA.
⁴ Iiiconsulting, LLC, Conway, SC 29526, USA.
⁵ Daicel Chiral Technologies, 1475 Dunwoody Dr. Suite 310, West Chester, PA 19380, USA.

PMID: 37451696
DOI: 10.1093/chromsci/bmad048

Abstract

An optimized method employing chiral supercritical fluid chromatography with diode array UV-VIS detection has been developed for the quantitative analysis of nicotine and nornicotine enantiomer distributions. The method parameters that were optimized included: column type (stationary phases, Chiralpak IG-3), column temperature (40°C), modifier types and concentration (isopropyl alcohol, 10%), additive types and concentrations (diethylamine, 0.2%), elution times (<6 min, flow rate 3 mL/min) and resolution factor (>1.2). These optimized conditions led to nicotine and nornicotine enantiomer detection limits of ~5 ng/μL with accompanying %RSD values of <2% from the analyses of commercially available nicotine-containing formulations.