Role of mass effect on neuronal iron deposition after intracerebral hemorrhage

Yuhua Gong; Jia Deng; Yingqing Wu; Xiaoyun Xu; Zongkun Hou; Shilei Hao; Bochu Wang

doi:10.1016/j.expneurol.2023.114475

Role of mass effect on neuronal iron deposition after intracerebral hemorrhage

Exp Neurol. 2023 Oct:368:114475. doi: 10.1016/j.expneurol.2023.114475. Epub 2023 Jul 13.

Authors

Yuhua Gong¹, Jia Deng², Yingqing Wu³, Xiaoyun Xu³, Zongkun Hou⁴, Shilei Hao⁵, Bochu Wang⁶

Affiliations

¹ Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China; School of Smart Health, Chongqing College of Electronic Engineering, Chongqing 401331, China.
² College of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, China. Electronic address: jiadeng2011@hotmail.com.
³ Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China.
⁴ Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Cellular Immunotherapy Engineering Research Center of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, China; Key Laboratory of Biology and Medical Engineering, Immune Cells and Antibody Engineering Research Center of Guizhou Province, School of Biology and Engineering, Guizhou Medical University, Guiyang 550025, China.
⁵ Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China. Electronic address: shilei_hao@cqu.edu.cn.
⁶ Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China. Electronic address: wangbc2000@126.com.

PMID: 37451583
DOI: 10.1016/j.expneurol.2023.114475

Abstract

Mass effect after intracerebral hemorrhage (ICH) not only mechanically induces the brain damage, but also influences the progress of secondary brain damage. However, the influence of mass effect on the iron overload after ICH is still unclear. Here, a fixed volume of ferrous chloride solution and different volumes of poly(N-isopropylacrylamide) (PNIPAM) hydrogel were co-injected into the right basal ganglia of rats to establish the ICH model with certain degree of iron deposition but different degrees of mass effect. We found that mass effect significantly increased the iron deposition on neuronal cells at 6 h after ICH in a volume-dependent manner. Furthermore, the upregulation of Piezo-2, divalent metal transporter 1 (DMT1), transferrin receptor (TfR), and ferroptosis expressions were noted as the increase of mass effect. In addition, the pERK1/2 inhibitor PD98059 treated ICH rats reversed the upregulation of iron uptake protein and ferroptosis. Our findings revealed the relationship between mass effect and the iron uptake and ferroptosis, which are benefit to understand the brain damage process after ICH.

Keywords: Ferroptosis; Intracerebral hemorrhage; Iron overload; Mass effect.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / metabolism
Brain Injuries* / metabolism
Cerebral Hemorrhage / complications
Cerebral Hemorrhage / metabolism
Iron / metabolism
Iron Overload* / complications
Iron Overload* / metabolism
Rats
Rats, Sprague-Dawley

Substances

Iron