Detection of Protein-Protein Interactions Using Glutathione-S-Transferase (GST) Pull-Down Assay Technique

Prabu Gnanasekaran; Hanu R Pappu

doi:10.1007/978-1-0716-3327-4_10

Detection of Protein-Protein Interactions Using Glutathione-S-Transferase (GST) Pull-Down Assay Technique

Methods Mol Biol. 2023:2690:111-115. doi: 10.1007/978-1-0716-3327-4_10.

Authors

Prabu Gnanasekaran¹, Hanu R Pappu²

Affiliations

¹ Department of Plant Pathology, Washington State University, Pullman, WA, USA.
² Department of Plant Pathology, Washington State University, Pullman, WA, USA. hrp@wsu.edu.

PMID: 37450141
DOI: 10.1007/978-1-0716-3327-4_10

Abstract

Pull-down assay is a technique to analyze direct protein-protein interaction under in vitro condition. Also, this technique is appropriate for investigating the direct interaction between two purified proteins. Glutathione-s-transferase (GST) protein is a widely used affinity tag for affinity purification. In this chapter, we explain the widely used GST pull-down assay to identify the protein-protein interaction between purified proteins.

Keywords: Affinity purification; Direct protein; Glutathione-s-transferase; Pull-down; protein interaction.

MeSH terms

Chromatography, Affinity / methods
Escherichia coli / metabolism
Glutathione / metabolism
Glutathione Transferase* / genetics
Glutathione Transferase* / metabolism
Proteins* / metabolism
Recombinant Fusion Proteins / metabolism

Substances

Glutathione Transferase
Proteins
Glutathione
Recombinant Fusion Proteins