Yeast Two-Hybrid Technique to Identify Protein-Protein Interactions

Prabu Gnanasekaran; Hanu R Pappu

doi:10.1007/978-1-0716-3327-4_1

Yeast Two-Hybrid Technique to Identify Protein-Protein Interactions

Methods Mol Biol. 2023:2690:1-8. doi: 10.1007/978-1-0716-3327-4_1.

Authors

Prabu Gnanasekaran¹, Hanu R Pappu²

Affiliations

¹ Department of Plant Pathology, Washington State University, Pullman, WA, USA.
² Department of Plant Pathology, Washington State University, Pullman, WA, USA. hrp@wsu.edu.

PMID: 37450132
DOI: 10.1007/978-1-0716-3327-4_1

Abstract

Protein-protein interactions are specific and direct physical contact between two or more proteins, and the interaction involves hydrogen bonding, electrostatic forces, and hydrophobic forces. Majority of biological processes in the living cell are executed by proteins, and any particular protein function is regulated by numerous other proteins. Thus, knowledge of protein-protein interaction is necessary to understand the biological processes. In this chapter, we explain the widely used yeast two-hybrid assay to identify the protein-interacting partners.

Keywords: Bait; GAL4; Prey; Protein–protein interaction; Y2H; Yeast.

MeSH terms

Protein Interaction Mapping / methods
Proteins* / metabolism
Saccharomyces cerevisiae*
Two-Hybrid System Techniques

Substances

Proteins