The Protocol of Circulating Tumor Cell Detection

Methods Mol Biol. 2023:2695:1-8. doi: 10.1007/978-1-0716-3346-5_1.

Abstract

The detection of CTCs is related to the development of tumors and can be used in medical fields such as early diagnosis, postoperative evaluation, monitoring treatment, and predicting disease prognosis. This article focuses on the entire process of CTC detection, including negative enrichment isolation and immunofluorescence in situ hybridization detection.

Keywords: Circulating tumor cell; FISH; Negative enrichment.

MeSH terms

  • Biomarkers, Tumor / genetics
  • Fluorescent Antibody Technique
  • Humans
  • Neoplastic Cells, Circulating* / pathology

Substances

  • Biomarkers, Tumor