Co-occurrence of marine and freshwater phycotoxins in oysters, and analysis of possible predictors for management

Sarah K D Pease; Todd A Egerton; Kimberly S Reece; Marta P Sanderson; Michelle D Onofrio; Evan Yeargan; Adam Wood; Amanda Roach; I-Shuo Wade Huang; Gail P Scott; Allen R Place; Amy M Hayes; Juliette L Smith

doi:10.1016/j.toxcx.2023.100166

Co-occurrence of marine and freshwater phycotoxins in oysters, and analysis of possible predictors for management

Toxicon X. 2023 Jun 16:19:100166. doi: 10.1016/j.toxcx.2023.100166. eCollection 2023 Sep.

Affiliations

¹ Virginia Institute of Marine Science, William & Mary, P.O. Box 1346, Gloucester Point, VA, 23062, USA.
² Division of Shellfish Safety and Waterborne Hazards, Virginia Department of Health, Norfolk, VA, 23510, USA.
³ Institute of Marine and Environmental Technology, University of Maryland, Center for Environmental Sciences, Baltimore, MD, 21202, USA.
⁴ Public Health Toxicology Program, Virginia Department of Health, Richmond, VA, 23219, USA.

Abstract

Oysters (Crassostrea virginica) were screened for 12 phycotoxins over two years in nearshore waters to collect baseline phycotoxin data and to determine prevalence of phycotoxin co-occurrence in the commercially and ecologically-relevant species. Trace to low concentrations of azaspiracid-1 and -2 (AZA1, AZA2), domoic acid (DA), okadaic acid (OA), and dinophysistoxin-1 (DTX1) were detected, orders of magnitude below seafood safety action levels. Microcystins (MCs), MC-RR and MC-YR, were also found in oysters (maximum: 7.12 μg MC-RR/kg shellfish meat wet weight), warranting consideration of developing action levels for freshwater phycotoxins in marine shellfish. Oysters contained phycotoxins that impair shellfish health: karlotoxin1-1 and 1-3 (KmTx1-1, KmTx1-3), goniodomin A (GDA), and pectenotoxin-2 (PTX2). Co-occurrence of phycotoxins in oysters was common (54%, n = 81). AZAs and DA co-occurred most frequently of the phycotoxins investigated that are a concern for human health (n = 13) and PTX2 and KmTxs co-occurred most frequently amongst the phycotoxins of concern for shellfish health (n = 9). Various harmful algal bloom (HAB) monitoring methods and tools were assessed for their effectiveness at indicating levels of phycotoxins in oysters. These included co-deployed solid phase adsorption toxin tracking (SPATT) devices, toxin levels in particulate organic matter (POM, >1.5 μm) and whole water samples and cell concentrations from water samples as determined by microscopy and quantitative real-time PCR (qPCR). The dominant phycotoxin varied between SPATTs and all other phycotoxin sample types, and out of the 11 phycotoxins detected in oysters, only four and seven were detected in POM and whole water respectively, indicating phycotoxin profile mismatch between ecosystem compartments. Nevertheless, there were correlations between DA in oysters and whole water (simple linear regression [LR]: R² = 0.6, p < 0.0001, n = 40), and PTX2 in oysters and SPATTs (LR: R² = 0.3, p = 0.001, n = 36), providing additional monitoring tools for these phycotoxins, but oyster samples remain the best overall indicators of seafood safety.

Keywords: Azaspiracid; Domoic acid; Karlotoxin; Microcystin; Okadaic acid; Pectenotoxin.