Synthesis of Cu2+ doped biochar and its inactivation performance of Microcystis aeruginosa: Significance of synergetic effect

Abstract

The harmful cyanobacteria bloom is frequently occurring in the aquatic environment and poses a tremendous threat to both aquatic organisms and ecological function. In this study, a series of Cu²⁺ doped biochar (BC) composites (Cu-BCs) with different loading ratios (0.1 %-5 wt %) (Cu-BC-0.1/0.5/1/2.5/5) was successfully fabricated through a one-step adsorption method for in-situ inactivation of Microcystis aeruginosa and simultaneous removal of microcystin-LR (MC-LR). Compared with the single BC/CuSO₄ and other Cu-BCs composites, the Cu-BC-2.5 exhibited the best algae inactivation performance with the lowest 72 h medium effective concentration (EC₅₀) value of 0.34 mg/L and highest chlorophyll α degradation efficiency of 8.31 g/g. Notably, the as-prepared Cu-BC-2.5 maintained good inactivation performance in the near-neutral pH (6.5-8.5), and the presence of humic acid and salts such as Na₂CO₃ and NaCl. The outstanding inhibitory effect of the Cu-BC-2.5 could be explained by the synergetic effect between biochar and Cu²⁺, which greatly elevated reactive oxygen species (ROS) intensity and in turn led to severe membrane damage and collapse of the antioxidant system. Additionally, the Cu-BC-2.5 could simultaneously remove the released microcystin-LR (MC-LR) throughout the inactivation process and prevent secondary pollution, thus offering a new insight into the alleviation of harmful cyanobacteria in aquatic environment.

Keywords: Biochar; Copper; Mechanism; Microcystis aeruginosa; Toxicity.