Primary Retinal Cell Cultures as a Model to Study Retina Biology

Germán A Michelis; Luis E Politi; S Patricia Becerra

doi:10.1007/978-3-031-27681-1_82

Primary Retinal Cell Cultures as a Model to Study Retina Biology

Adv Exp Med Biol. 2023:1415:565-569. doi: 10.1007/978-3-031-27681-1_82.

Authors

Germán A Michelis^{1

2}, Luis E Politi², S Patricia Becerra³

Affiliations

¹ Section of Protein Structure and Function, LRCMB, NEI-NIH, Bethesda, MD, USA.
² Department of Biology, Pharmacy and Biochemistry, Instituto de Investigaciones Bioquímicas (INIBIBB), Universidad Nacional del Sur (UNS)-CONICET, Bahía Blanca, Argentina.
³ Section of Protein Structure and Function, LRCMB, NEI-NIH, Bethesda, MD, USA. becerras@nei.nih.gov.

PMID: 37440087
DOI: 10.1007/978-3-031-27681-1_82

Abstract

Since its inception, primary retinal cultures have been an in vitro tool for modeling the in vivo environment of the retina for biological studies on development and disease. They offer simple and controlled experimental approaches when compared to in vivo models. In this review we highlight the strengths and weaknesses of primary retinal culture models, and the features of dispersed retinal cell cultures.

Keywords: Cell lines; In vitro models; In vivo models; Organoids; Primary cultures; Retina.

Publication types

Review

MeSH terms

Biology
Cell Culture Techniques*
Cell Differentiation
Neurons
Retina*