Methods for In Vivo Characterization of Proteostasis in the Mouse Retina

Abstract

An increasing number of studies connect inherited and age-related retinal degenerations with changes in the regulation of proteostasis. Here, we describe technical aspects of existing assays allowing to assess the status of the ubiquitin-proteasome system (UPS), changes in autophagy, and protein translation in mouse retina in vivo. These methods are helpful for the development and testing approaches to modulate proteostasis and delay vision loss.

Keywords: Autophagy; Protein translation; Proteostasis; Retinal degeneration; Ubiquitin-proteasome system.