Applied Biosystems' GlobalFiler™ PCR Amplification Kit

Georgia Williams; Megan M Foley; Kelly L Knight

doi:10.1007/978-1-0716-3295-6_15

Applied Biosystems' GlobalFiler™ PCR Amplification Kit

Methods Mol Biol. 2023:2685:241-252. doi: 10.1007/978-1-0716-3295-6_15.

Authors

Georgia Williams¹, Megan M Foley², Kelly L Knight³

Affiliations

¹ Forensic Science Program, George Mason University, Fairfax, VA, USA. gwilli25@gmu.edu.
² Department of Forensic Sciences, The George Washington University, Washington, DC, USA.
³ Forensic Science Program, George Mason University, Fairfax, VA, USA.

PMID: 37439986
DOI: 10.1007/978-1-0716-3295-6_15

Abstract

The GlobalFiler™ PCR Amplification Kit is one of the most sensitive kits that exist today that makes the PCR amplification of human DNA possible. PCR amplification using this specific kit makes millions of copies of 24 specific target sequences in the DNA, called markers or loci. This kit is a 6-dye, short tandem repeat (STR) multiplex assay kit that has a synthetic mix of primers and single-stranded oligonucleotides that are combined with DNA samples and then subjected to 29 or 30 cycles of denaturing, annealing, and extension, as per laboratory protocol. Methods for instrument operation will vary depending on the thermal cycler instrument model that is used. Nevertheless, the GlobalFiler™ PCR Amplification Kit has proven to be a very useful tool to DNA analysts, amplifying extremely low quantities of DNA, making it possible to detect partial, if not full, genetic profiles from a wide range of sample types. This chapter discusses the typical preparation and PCR amplification of human forensic DNA samples, using the GlobalFiler™ PCR Amplification Kit.

Keywords: Amplification; CODIS; DNA polymerase; Forensic Genetics; GlobalFiler™; Loci; Multiplex; PCR; STR; ThermoFisher.

MeSH terms

DNA / genetics
DNA Fingerprinting* / methods
DNA Primers / genetics
Humans
Microsatellite Repeats* / genetics
Polymerase Chain Reaction / methods

Substances

DNA
DNA Primers