Quantitation of DNA Using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit

Kelly L Knight; Angelina Mauriello; Georgia Williams

doi:10.1007/978-1-0716-3295-6_11

Quantitation of DNA Using the Applied Biosystems Quantifiler^® Trio DNA Quantification Kit

Methods Mol Biol. 2023:2685:175-188. doi: 10.1007/978-1-0716-3295-6_11.

Authors

Kelly L Knight¹, Angelina Mauriello², Georgia Williams²

Affiliations

¹ Forensic Science Program, George Mason University, Fairfax, VA, USA. kknight6@gmu.edu.
² Forensic Science Program, George Mason University, Fairfax, VA, USA.

PMID: 37439982
DOI: 10.1007/978-1-0716-3295-6_11

Abstract

Following the isolation of deoxyribonucleic acid (DNA) from biological samples, the quantitation of amplifiable human DNA is a critical next step in the process of DNA analysis. The Quantifiler^® Trio kit provides a simple way to accurately estimate the quantity of human and male DNA with concentrations as low as 5 pg/μL or less. Not only can the Quantifiler^® Trio kit determine the quantity of human DNA present, but it can also give an indication of the quality of the sample, which is essential information to have in the decision-making process regarding any downstream testing being done. In this chapter, we describe how to prepare and process quantitation reactions using the Quantifiler^® Trio kit. We also provide basic information on how to interpret the results.

Keywords: DNA degradation; DNA inhibition; DNA quantitation; Degradation index; Forensic DNA; Quantification; Quantifiler Trio; Quantitative real-time PCR; RT-qPCR; qPCR.

MeSH terms

DNA Fingerprinting* / methods
DNA* / analysis
DNA* / genetics
Humans
Male
Microsatellite Repeats

Substances

DNA