Enhanced mixotrophic production of lutein and lipid from potential microalgae isolate Chlorella sorokiniana C16

Anil Kumar Patel; Akash Pralhad Vadrale; Reeta-Rani Singhania; Chiu-Wen Chen; Jo Shu Chang; Cheng-Di Dong

doi:10.1016/j.biortech.2023.129477

Enhanced mixotrophic production of lutein and lipid from potential microalgae isolate Chlorella sorokiniana C16

Bioresour Technol. 2023 Oct:386:129477. doi: 10.1016/j.biortech.2023.129477. Epub 2023 Jul 10.

Authors

Anil Kumar Patel¹, Akash Pralhad Vadrale², Reeta-Rani Singhania¹, Chiu-Wen Chen³, Jo Shu Chang⁴, Cheng-Di Dong⁵

Affiliations

¹ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Centre for Energy and Environmental Sustainability, Lucknow 226 029, Uttar Pradesh, India.
² Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan.
³ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan.
⁴ Research Center for Smart Sustainable Circular Economy, Tunghai University, Taiwan; Department of Chemical and Materials Engineering, Tunghai University, Taiwan; Department of Chemical Engineering, National Cheng Kung University, Taiwan.
⁵ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan. Electronic address: cddong@nkust.edu.tw.

PMID: 37437816
DOI: 10.1016/j.biortech.2023.129477

Abstract

The current work aims to isolate high lutein-producing microalgae and maximize lutein production under a sustainable lutein-lipid biorefinery scheme. Lutein reduces retinitis, macular degeneration risk and improves eye health. An effective bioprocess design optimized nutrients, temperature, light, and salinity for biomass and lutein yield enhancement. 3X macro/micronutrients maximally enhanced biomass and lutein yields, 5.2 g/Land 71.13 mg/L. Temperature 32 °C exhibited maximum 17.4 mg/g lutein content and 10 k lux was most favorable for growth and lutein yield (15.47 mg/g). A 25% seawater addition led maximum of 21-27% lipid that could be used for biodiesel. Isolate was identified as Chlorella sorokiniana C16, which exhibited one of the highest lutein yields reported among recent studies, positioning it as a promising candidate for commercial lutein production. This study provides valuable insights into an effective bioprocess design and highlights the C16 strain potential as a sustainable platform for high-value lutein production under a biorefinery scheme.

Keywords: Bioprocess design; Chlorella sorokiniana C16; Lutein; Microalgae; Sustainable biorefinery.

MeSH terms

Biomass
Chlorella*
Lipids
Lutein
Microalgae*

Substances

Lutein
Lipids