Enhancing therapeutic effects of murine cancer vaccine by reshaping gut microbiota with Lactobacillus rhamnosus GG and jujube powder

Nan Jing; Luoyang Wang; Huiren Zhuang; Guoqiang Jiang; Zheng Liu

doi:10.3389/fimmu.2023.1195075

Enhancing therapeutic effects of murine cancer vaccine by reshaping gut microbiota with Lactobacillus rhamnosus GG and jujube powder

Front Immunol. 2023 Jun 26:14:1195075. doi: 10.3389/fimmu.2023.1195075. eCollection 2023.

Authors

Nan Jing^{1

2}, Luoyang Wang³, Huiren Zhuang^{1

2}, Guoqiang Jiang^{1

2}, Zheng Liu^{1

2}

Affiliations

¹ Department of Chemical Engineering, Tsinghua University, Beijing, China.
² Key Lab of Industrial Biocatalysis, Ministry of Education, Tsinghua University, Beijing, China.
³ School of Basic Medicine, Qingdao University, Qingdao, China.

Abstract

Cancer vaccines have gained widespread attention in recent years as an emerging treatment for tumors. However, most therapeutic cancer vaccines have failed in phase III clinical trials due to faint clinical benefits. In this study, we funded that a specific synbiotic composing Lactobacillus rhamnosus GG (LGG) and jujube powder significantly enhanced the therapeutic effects of whole cells cancer vaccine in MC38 cancer cells bearing-mouse. The utilization of LGG increased the abundance of Muribaculaceae, which is conductive to an enhanced anti-tumor effect, but reduced microbial α-diversity. The use of jujube nursed probiotic microorganisms in Lachnospiaceae and enriched microbial diversity, as indicated by increased Shannon and Chao index. The reshaped gut microbiota by this synbiotic improved lipid metabolism conductive to intensified infiltration of CD8⁺ T cells in the tumor microenvironment and enhanced the potency of above-mentioned cancer vaccine. These encouraging findings are helpful for further efforts towards enhancing the therapeutic effects of cancer vaccines through nutritional intervention.

Keywords: cancer vaccines; gut microbiota; immunotherapy; nutritional intervention; synbiotic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CD8-Positive T-Lymphocytes
Cancer Vaccines*
Gastrointestinal Microbiome*
Lacticaseibacillus rhamnosus*
Mice
Neoplasms*
Powders
Ziziphus*

Substances

Cancer Vaccines
Powders

Grants and funding

This work was funded by Xinjiang Tianchen Zhihui Information Technology Co. Ltd under project contract No. 20202001227. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication.