An ultrasensitive aptasensor for exosomes detection based on biotin-streptavidin and MXenes

Zhangmin Wang; Ze Zhang; Hongzhi Pan; Dong Chang

doi:10.1016/j.ab.2023.115233

An ultrasensitive aptasensor for exosomes detection based on biotin-streptavidin and MXenes

Anal Biochem. 2023 Sep 1:676:115233. doi: 10.1016/j.ab.2023.115233. Epub 2023 Jul 9.

Authors

Zhangmin Wang¹, Ze Zhang², Hongzhi Pan³, Dong Chang⁴

Affiliations

¹ Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, China; Shanghai Center for Clinical Laboratory, Shanghai, 200126, China.
² Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, China.
³ Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201399, China. Electronic address: panhongzhi@163.com.
⁴ Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, China. Electronic address: changdongydy@163.com.

PMID: 37433362
DOI: 10.1016/j.ab.2023.115233

Abstract

Exosomes derived from lung cancer typically contain the genetic information of the donor cells. Therefore, exosomes contribute to early diagnosis, treatment effectiveness evaluation, and prognosis assessment of cancer. Based on the biotin-streptavidin system and MXenes nanomaterial, a dual-effect amplification method had been developed to construct an ultrasensitive colorimetric aptasensor for detecting exosomes. MXenes can enhance the loading of aptamer and biotin as the high specific surface area. Biotin-streptavidin system can increase the amount of horseradish peroxidase-linked (HRP-linked) streptavidin, considerably boosting the color signal of the aptasensor. The proposed colorimetric aptasensor exhibited excellent sensitivity, with a detection limit of 42 particles μL^-1 and a linear range of 10² to 10⁷ particles μL^-1. The constructed aptasensor showed satisfactory reproducibility, stability, and selectivity, confirming the promising application of exosomes in clinical cancer detection.

Keywords: Colorimetric aptasensor; Dual-effect amplification method; Exosomes detection; Lung cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques* / methods
Biotin
Exosomes*
Limit of Detection
Reproducibility of Results
Streptavidin

Substances

Streptavidin
Biotin
Aptamers, Nucleotide