Protocol for mechanochemistry-guided assembly strategy for enzyme encapsulation using covalent organic frameworks

Rui Gao; Xiaoxue Kou; Rongwei He; Yujiang Shen; Lihong Guo; Hao Wang; Siming Huang; Guosheng Chen; Gangfeng Ouyang

doi:10.1016/j.xpro.2023.102421

Protocol for mechanochemistry-guided assembly strategy for enzyme encapsulation using covalent organic frameworks

STAR Protoc. 2023 Sep 15;4(3):102421. doi: 10.1016/j.xpro.2023.102421. Epub 2023 Jul 9.

Authors

Rui Gao¹, Xiaoxue Kou¹, Rongwei He¹, Yujiang Shen¹, Lihong Guo¹, Hao Wang¹, Siming Huang², Guosheng Chen³, Gangfeng Ouyang¹

Affiliations

¹ MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.
² Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, the NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, China. Electronic address: huangsm@gzhmu.edu.cn.
³ MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: chengsh39@mail.sysu.edu.cn.

Abstract

Enzyme immobilization into porous frameworks is an emerging strategy for enhancing the stability of dynamic conformation and prolonging the lifespan of enzymes. Here, we present a protocol for a de novo mechanochemistry-guided assembly strategy for enzyme encapsulation using covalent organic frameworks. We describe steps for mechanochemical synthesis, enzyme loading measurements, and material characterizations. We then detail evaluations of biocatalytic activity and recyclability. For complete details on the use and execution of this protocol, please refer to Gao et al. (2022).¹.

Keywords: Chemistry; Material sciences; X-ray Crystallography.

MeSH terms

Metal-Organic Frameworks*
Porosity

Substances

Metal-Organic Frameworks