Viral load quantitation at the point-of-care with shaken digital droplet RT-LAMP

Lab Chip. 2023 Jul 25;23(15):3479-3486. doi: 10.1039/d3lc00096f.

Abstract

Viral load quantitation is useful in clinical point-of-care settings to assess the status of patients with infectious disease, track response to treatment, and estimate infectiousness. However, existing methods for quantitating viral loads are complex and difficult to integrate into these settings. Here, we describe a simple, instrument-free approach for viral load quantitation suitable for point-of-care use. We develop a shaken digital droplet assay that can quantitate SARS-CoV2 with sensitivity comparable to gold standard qPCR.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • COVID-19*
  • Humans
  • Point-of-Care Systems*
  • RNA, Viral / analysis
  • SARS-CoV-2
  • Sensitivity and Specificity
  • Viral Load / methods

Substances

  • RNA, Viral

Supplementary concepts

  • LAMP assay