Single-Cell Micropatterning by Non-fouling Hydrogels

Wengang Liu; Ting-Hsuan Chen; Jiandong Ding

doi:10.1007/978-1-0716-3323-6_6

Single-Cell Micropatterning by Non-fouling Hydrogels

Methods Mol Biol. 2023:2689:65-70. doi: 10.1007/978-1-0716-3323-6_6.

Authors

Wengang Liu¹, Ting-Hsuan Chen², Jiandong Ding³

Affiliations

¹ Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China.
² Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China. thchen@cityu.edu.hk.
³ State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, China.

PMID: 37430047
DOI: 10.1007/978-1-0716-3323-6_6

Abstract

Micropatterned substrate is a unique method for studying cell biology at the single-cell level. Using photolithography to create binary patterns of cell-adherent peptide surrounding by non-fouling cell-repellent poly(ethylene glycol) (PEG) hydrogel, this patterning method allows for controlling cell attachment with desired sizes and shapes up to 19 days. Here we provide the detailed procedure of fabrication for such patterns. This method will allow monitoring of prolonged reaction of single cells such as cell differentiation upon induction or time-resolved apoptosis stimulated by drug molecules for cancer treatment.

Keywords: Photolithography; Poly(ethylene glycol) hydrogel; Single-cell micropatterning.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis*
Cell Differentiation
Cell-Matrix Junctions*
Hydrogels
Polyethylene Glycols

Substances

Hydrogels
Polyethylene Glycols