Comprehensive analysis of transcriptional data on seed germination of two maize inbred lines under low-temperature conditions

Yinchao Zhang; Peng Liu; Chaoying Zou; Zhong Chen; Guangsheng Yuan; Shibin Gao; Guangtang Pan; Yaou Shen; Langlang Ma

doi:10.1016/j.plaphy.2023.107874

Comprehensive analysis of transcriptional data on seed germination of two maize inbred lines under low-temperature conditions

Plant Physiol Biochem. 2023 Aug:201:107874. doi: 10.1016/j.plaphy.2023.107874. Epub 2023 Jul 7.

Authors

Yinchao Zhang¹, Peng Liu², Chaoying Zou², Zhong Chen², Guangsheng Yuan², Shibin Gao², Guangtang Pan², Yaou Shen³, Langlang Ma⁴

Affiliations

¹ State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
² State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
³ State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: shenyaou@sicau.edu.cn.
⁴ State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: malanglang@sicau.edu.cn.

PMID: 37429215
DOI: 10.1016/j.plaphy.2023.107874

Abstract

Seed germination directly affect maize yield and grain quality. Low-temperature reduces maize yield by affecting seed germination and seedling growth. However, the molecular mechanism of maize seed germination under low-temperature remains unclear. In this study, the transcriptome data of two maize inbred lines SCL127 (chilling-sensitive) and SCL326 (chilling-tolerant) were analyzed at five time points (0 H, 4 H, 12 H, 24 H, and 48 H) under low-temperature conditions. Through the comparison of SCL127-0 H-vs-SCL326-0 H (Group I), SCL127-4 H-vs-SCL326-4 H (Group Ⅱ), SCL127-12 H-vs-SCL326-12 H (Group Ⅲ), SCL127-24 H-vs-SCL326-24 H (Group Ⅳ), and SCL127-48 H-vs SCL326-48 H (Group Ⅴ), a total of 8,526 differentially expressed genes (DEGs) were obtained. Weighted correlation network analysis revealed that Zm00001d010445 was the hub gene involved in seed germination under low-temperature conditions. Zm00001d010445-based association analysis showed that Hap Ⅱ (G) was the excellent haplotype for seed germination under low-temperature conditions. These findings provide a new perspective for the study of the genetic architecture of maize tolerance to low-temperature and contribute to the cultivation of maize varieties with low-temperature tolerance.

Keywords: Hub genes; Low-temperature; Maize; RNA-Seq; Seed germination.

MeSH terms

Germination* / genetics
Seedlings
Seeds / genetics
Temperature
Zea mays* / genetics