The use of flow cytometry and fluorescence-activated cell sorting to roughly separate subpopulations of cells in Schmidtea mediterranea is long established. In this chapter, we describe a method for the immunostaining-either single or double-of live planarian cells, using mouse monoclonal antibodies reactive against S. mediterranea plasma membrane antigens. This protocol allows to sort live cells according to their membrane signature, offering the possibility to further characterize the cell populations in S. mediterranea in a variety of downstream applications, like transcriptomics and cell transplantation, also at the single-cell level.
Keywords: Cell subpopulations; FACS; Flow cytometry; Hybridoma library; Live immunostaining; Monoclonal antibody; Plasma membrane antigens; Schmidtea mediterranea.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.