An integrative multi-omics analysis based on disulfidptosis-related prognostic signature and distinct subtypes of clear cell renal cell carcinoma

Dong Zhang; Xi Zhang; Zhanpeng Liu; Tian Han; Kai Zhao; Xinchi Xu; Xu Zhang; Xiaohan Ren; Chao Qin

doi:10.3389/fonc.2023.1207068

An integrative multi-omics analysis based on disulfidptosis-related prognostic signature and distinct subtypes of clear cell renal cell carcinoma

Front Oncol. 2023 Jun 23:13:1207068. doi: 10.3389/fonc.2023.1207068. eCollection 2023.

Authors

Dong Zhang¹, Xi Zhang¹, Zhanpeng Liu¹, Tian Han¹, Kai Zhao¹, Xinchi Xu¹, Xu Zhang¹, Xiaohan Ren¹, Chao Qin¹

Affiliation

¹ The State Key Lab of Reproductive, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Abstract

Background: The association between clear cell renal cell carcinoma (ccRCC) and disulfidoptosis remains to be thoroughly investigated.

Methods: We conducted multiple bioinformatics analyses, including prognostic analysis and cluster analysis, using R software. Additionally, we utilized Quantitative Real-time PCR to measure RNA levels of specific genes. The proliferation of ccRCC was assessed through CCK8 and colony formation assays, while the invasion and migration of ccRCC cells were evaluated using the transwell assay.

Results: In this study, utilizing data from multiple ccRCC cohorts, we identified molecules that contribute to disulfidoptosis. We conducted a comprehensive investigation into the prognostic and immunological roles of these molecules. Among the disulfidoptosis-related metabolism genes (DMGs), LRPPRC, OXSM, GYS1, and SLC7A11 exhibited significant correlations with ccRCC patient prognosis. Based on our signature, patients in different groups displayed varying levels of immune infiltration and different mutation profiles. Furthermore, we classified patients into two clusters and identified multiple functional pathways that play important roles in the occurrence and development of ccRCC. Given its critical role in disulfidoptosis, we conducted further analysis on SLC7A11. Our results demonstrated that ccRCC cells with high expression of SLC7A11 exhibited a malignant phenotype.

Conclusions: These findings enhanced our understanding of the underlying function of DMGs in ccRCC.

Keywords: SLC7A11; clear cell renal cell carcinoma; disulfidptosis; immunity; prognosis.

Grants and funding

This work was supported by the National Natural Science Foundation of China (grant number: 81972386) and Jiangsu Province Capability Improvement Project through Science, Technology and Education (grant number: ZDXK202219).