A chromosomal reference genome sequence for the malaria mosquito, Anopheles gambiae, Giles, 1902, Ifakara strain

Tibebu Habtewold; Martin Wagah; Mgeni Mohamed Tambwe; Sarah Moore; Nikolai Windbichler; George Christophides; Harriet Johnson; Haynes Heaton; Joanna Collins; Ksenia Krasheninnikova; Sarah E Pelan; Damon-Lee B Pointon; Ying Sims; James W Torrance; Alan Tracey; Marcela Uliano Da Silva; Jonathan Md Wood; Katharina von Wyschetzki; Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective; Shane A McCarthy; Daniel E Neafsey; Alex Makunin; Mara K N Lawniczak; Mara Lawniczak

doi:10.12688/wellcomeopenres.18854.1

A chromosomal reference genome sequence for the malaria mosquito, Anopheles gambiae, Giles, 1902, Ifakara strain

Wellcome Open Res. 2024 Mar 26:8:74. doi: 10.12688/wellcomeopenres.18854.1. eCollection 2023.

Authors

Tibebu Habtewold¹, Martin Wagah², Mgeni Mohamed Tambwe³, Sarah Moore^{3

4}, Nikolai Windbichler¹, George Christophides¹, Harriet Johnson⁵, Haynes Heaton⁶, Joanna Collins², Ksenia Krasheninnikova², Sarah E Pelan², Damon-Lee B Pointon², Ying Sims², James W Torrance², Alan Tracey², Marcela Uliano Da Silva², Jonathan Md Wood², Katharina von Wyschetzki²; Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective; Shane A McCarthy², Daniel E Neafsey^{7

8}, Alex Makunin^#², Mara K N Lawniczak^#², Mara Lawniczak²

Affiliations

¹ Department of Life Sciences, Imperial College London, London, UK.
² Tree of Life, Wellcome Sanger Institute, Hinxton, UK.
³ Vector Control Product Testing Unit, Ifakara Health institute, Bagamoyo, Tanzania.
⁴ Vector Biology Unit, Swiss Tropical and Public Health Institute, Bagamoyo, Tanzania.
⁵ Scientific Operations, Wellcome Sanger Institute, Hinxton, UK.
⁶ CSSE, Auburn University, Auburn, Alabama, USA.
⁷ Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, USA.
⁸ Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA.

^# Contributed equally.

Abstract

We present a genome assembly from an individual female Anopheles gambiae (the malaria mosquito; Arthropoda; Insecta; Diptera; Culicidae), Ifakara strain. The genome sequence is 264 megabases in span. Most of the assembly is scaffolded into three chromosomal pseudomolecules with the X sex chromosome assembled. The complete mitochondrial genome was also assembled and is 15.4 kilobases in length.

Keywords: African malaria mosquito; Anopheles gambiae; chromosomal; genome sequence.

Abstract

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