Screening of wheat grains enriched with wall-bound phenolic compounds

Yi-Lin Gong; Jin-Ying Gou

doi:10.1016/j.mex.2023.102245

Screening of wheat grains enriched with wall-bound phenolic compounds

MethodsX. 2023 Jun 7:10:102245. doi: 10.1016/j.mex.2023.102245. eCollection 2023.

Authors

Yi-Lin Gong¹, Jin-Ying Gou^{1

2}

Affiliations

¹ School of Life Sciences, Fudan University, Shanghai, 200438, China.
² College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.

Abstract

Phenolic compounds are dominant antioxidant factors in whole grains and are essential quality traits in future breeding programs. We proposed a robust set of methods for extraction, screening, and quantitative analysis of soluble and wall-bound (WB) phenolic compounds from fine powder and fine powder products using a 96 Wells UV Flat Bottom and subsequent UHPLC-DAD validation of candidate samples. The plate-UHPLC strategy significantly simplifies the screening of phenolic-enriched grains, reduces the screening cost, saves harmful organic chemicals, and contributes to developing novel health-promoting varieties.

Keywords: Ferulic acid; Liquid chromatography; Screening phenolic-enriched grains; Ultraviolet spectrophotometry; Wheat.