In Vitro Models to Evaluate Molecular Permeability of the Kidney Filtration Barrier

Dan Wang; Nicholas Ferrell

doi:10.1007/978-1-0716-3179-9_4

In Vitro Models to Evaluate Molecular Permeability of the Kidney Filtration Barrier

Methods Mol Biol. 2023:2664:41-53. doi: 10.1007/978-1-0716-3179-9_4.

Authors

Dan Wang¹, Nicholas Ferrell²

Affiliations

¹ Department of Internal Medicine, Division of Nephrology, Ohio State University Wexner Medical Center, Columbus, OH, USA.
² Department of Internal Medicine, Division of Nephrology, Ohio State University Wexner Medical Center, Columbus, OH, USA. ferrell.61@osu.edu.

PMID: 37423981
DOI: 10.1007/978-1-0716-3179-9_4

Abstract

The glomerular basement membrane (GBM) is an important component of the kidney filtration barrier. The ability to evaluate the molecular transport properties of the GBM and determining how changes in the structure, composition, and mechanical properties of the GBM regulate its size selective transport properties may provide additional insight into glomerular function. This chapter details a method for making in vitro models of the glomerular filtration barrier using animal-derived decellularized glomeruli. FITC-labelled Ficoll is used as a filtration probe to evaluate the molecular transport properties during passive diffusion and under applied pressure. These systems can serve as a platform to evaluate the molecular permeability of basement membrane systems using conditions that simulate normal or pathophysiological conditions.

Keywords: Ficoll; Glomerular basement membrane; Kidney filtration; Molecular permeability.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Basement Membrane / physiology
Glomerular Filtration Barrier*
Glomerular Filtration Rate
Kidney Glomerulus*
Permeability