Red-emitting fluorescent probe for hydrogen sulfide detection and its applications in food freshness determination and in vivo bioimaging

Zhuye Shang; Qingtao Meng; Dihua Tian; Yue Wang; Zexi Zhang; Zhiqiang Zhang; Run Zhang

doi:10.1016/j.foodchem.2023.136701

Red-emitting fluorescent probe for hydrogen sulfide detection and its applications in food freshness determination and in vivo bioimaging

Food Chem. 2023 Nov 30:427:136701. doi: 10.1016/j.foodchem.2023.136701. Epub 2023 Jun 24.

Authors

Zhuye Shang¹, Qingtao Meng², Dihua Tian³, Yue Wang¹, Zexi Zhang³, Zhiqiang Zhang⁴, Run Zhang⁵

Affiliations

¹ School of Chemical Engineering, University of Science and Technology Liaoning, Anshan, Liaoning Province, 114051, PR China.
² School of Chemical Engineering, University of Science and Technology Liaoning, Anshan, Liaoning Province, 114051, PR China; Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan, Liaoning Province 114051, PR China. Electronic address: qtmeng@ustl.edu.cn.
³ Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
⁴ School of Chemical Engineering, University of Science and Technology Liaoning, Anshan, Liaoning Province, 114051, PR China. Electronic address: zzq@ustl.edu.cn.
⁵ Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: r.zhang@uq.edu.au.

PMID: 37423045
DOI: 10.1016/j.foodchem.2023.136701

Abstract

We report the development of a red-emitting fluorescence probe (XDS) for hydrogen sulfide (H₂S) detection in biosystems, real-world food samples, and application of this probe for monitoring of H₂S production during food spoilage. The XDS probe is developed by coupling of coumarin derivative to rhodanic-CN through a H₂S responsive CC bond. Remarkable fluorescence quenching of XDS is observed as a result of the response to H₂S. Semi-quantitative detection of H₂S in three real-world water and two beer samples and monitoring of H₂S production during food spoilage in real-time by "naked-eye" and smartphone colorimetric analysis are then achieved using XDS as the probe. Moreover, XDS is low toxicity, allowing it being used for visualizing endogenous and exogenous H₂S in vivo in a mouse model. It is expected that the successful development of XDS could provide an effective tool for investigating the roles of H₂S in biomedical system and for future food safety evaluation.

Keywords: Bioimaging; Fluorescent probe; Food spoilage; Hydrogen sulfide.

MeSH terms

Animals
Beer / analysis
Colorimetry / methods
Fluorescent Dyes* / chemistry
HeLa Cells
Humans
Hydrogen Sulfide* / analysis
Mice
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
Hydrogen Sulfide