Review of covalent organic frameworks for enzyme immobilization: Strategies, applications, and prospects

Jie Feng; Qing-Yun Huang; Ce Zhang; Seeram Ramakrishna; Yu-Bin Dong

doi:10.1016/j.ijbiomac.2023.125729

Review of covalent organic frameworks for enzyme immobilization: Strategies, applications, and prospects

Int J Biol Macromol. 2023 Sep 1:248:125729. doi: 10.1016/j.ijbiomac.2023.125729. Epub 2023 Jul 7.

Authors

Jie Feng¹, Qing-Yun Huang², Ce Zhang², Seeram Ramakrishna³, Yu-Bin Dong⁴

Affiliations

¹ College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, China; Centre for Nanotechnology and Sustainability, Department of Mechanical Engineering, National University of Singapore, 117574 Singapore, Singapore.
² College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, China.
³ Centre for Nanotechnology and Sustainability, Department of Mechanical Engineering, National University of Singapore, 117574 Singapore, Singapore. Electronic address: seeram@nus.edu.sg.
⁴ College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, China. Electronic address: yubindong@sdnu.edu.cn.

PMID: 37422245
DOI: 10.1016/j.ijbiomac.2023.125729

Abstract

Efficient enzyme immobilization systems offer a promising approach for improving enzyme stability and recyclability, reducing enzyme contamination in products, and expanding the applications of enzymes in the biomedical field. Covalent organic frameworks (COFs) possess high surface areas, ordered channels, optional building blocks, highly tunable porosity, stable mechanical properties, and abundant functional groups, making them ideal candidates for enzyme immobilization. Various COF-enzyme composites have been successfully synthesized, with performances that surpass those of free enzymes in numerous ways. This review aims to provide an overview of current enzyme immobilization strategies using COFs, highlighting the characteristics of each method and recent research applications. The future opportunities and challenges of enzyme immobilization technology using COFs are also discussed.

Keywords: Application; Covalent organic frameworks; Enzyme immobilization.

Publication types

Review

MeSH terms

Metal-Organic Frameworks*
Porosity
Technology

Substances

Metal-Organic Frameworks