Triclosan (TCS) is an endocrine disruptor, which has been widely used in daily chemicals, resulting in the potential risk to the ecosystem and human health. Herein, a smartphone-integrated bimetallic nanozyme triple-emission fluorescence capillary imprinted sensing system was developed for ultrasensitive and intelligent visual microanalysis of TCS. Carbon dots (CDs) and bimetallic organic framework (MOF-(Fe/Co)-NH2) were used as fluorescence sources to synthesize nanozyme fluorescence molecularly imprinted polymer (MOF-(Fe/Co)-NH2@CDs@NMIP), which oxidized o-phenylenediamine to 2,3-diaminophenazine (OPDox), resulting in the derivation of a new fluorescence peak at 556 nm. In the existence of TCS, the fluorescence of MOF-(Fe/Co)-NH2 at 450 nm was restored, the fluorescence of OPDox at 556 nm was suppressed, and the CDs fluorescence of at 686 nm remained constant. The color of triple-emission fluorescence imprinted sensor varied from yellow to pink to purple to blue. The response efficiency (F450/F556/F686) of this sensing platform based on the capillary waveguide effect demonstrated a significant linear relationship toward the concentration of TCS ranged from 1.0 × 10-12 to 1.5 × 10-10 M with the LOD of 8.0 × 10-13 M. Compared with dual-emission capillary fluorescence sensor, this sensing system has higher sensitivity and richer visual color. Combined with the smartphone-integrated portable sensing platform, the color of fluorescence was transformed into an RGB value to calculate TCS concentration with the LOD of 9.6 × 10-13 M, providing a novel method for intelligent visual microanalysis (18 μL/time) of environmental pollutants.
Keywords: Bimetallic nanozyme; Intelligent visualization; Molecularly imprinted polymers; Triclosan; Triple-emission capillary fluorescence.
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