Quantification of indicator and pathogenic bacteria in manures and digestates from three agricultural biogas plants over a one-year period

Abstract

Interest in the conversion of manure in biogas via anaerobic digestion (AD) is growing, but questions remain about the biosafety of digestates. For a period of one year, we monitored the impact of three mesophilic agricultural biogas plants (BPs) mainly fed with pig manure (BP1, BP3) or bovine manure (BP2) on the physicochemical parameters, the composition of the microbial community and the concentration of bacteria (E. coli, enterococci, Salmonella, Campylobacter, Listeria monocytogenes, Clostridium perfringens, Clostridium botulinum and Clostridioides difficile). The BP2 digestate differed from those of the two other BPs with a higher nitrogen content, more total solids and greater abundance of Clostridia MBA03 and Disgonomonadacea. Persistence during digestion ranked from least to most, was: Campylobacter (1.6 to >2.9 log₁₀ reduction, according to the BP) < E. coli (1.8 to 2.2 log₁₀) < Salmonella (1.1 to 1.4 log₁₀) < enterococci (0.2 to 1.2 log₁₀) and C. perfringens (0.2 to 1 log₁₀) < L. monocytogenes (-1.2 to 1.6 log₁₀) < C. difficile and C. botulinum (≤0.5 log₁₀). No statistical link was found between the reduction in the concentration of the targeted bacteria and the physicochemical and operational parameters likely to have an effect (NH₃, volatile fatty acids and total solids contents, hydraulic retention time, presence of co-substrates), underlining the fact that the fate of the bacteria during mesophilic digestion depends on many interacting factors. The reduction in concentrations varied significantly over the sampling period, underlining the need for longitudinal studies to estimate the impact of AD on pathogenic microorganisms.

Keywords: Digestate; Fecal indicator bacteria; Manure; Mesophilic biogas plant; Microbial communities; Pathogenic bacteria.