Stress response and tolerance mechanisms of spirobudiclofen exposure based on multiomics in Panonychus citri (Acari: Tetranychidae)

Hongyan Wang; Tianrong Xin; Haifeng Wang; Kexin Wen; Yimeng Liu; Jing Wang; Zhiwen Zou; Ling Zhong; Bin Xia

doi:10.1016/j.isci.2023.107111

Stress response and tolerance mechanisms of spirobudiclofen exposure based on multiomics in Panonychus citri (Acari: Tetranychidae)

iScience. 2023 Jun 14;26(7):107111. doi: 10.1016/j.isci.2023.107111. eCollection 2023 Jul 21.

Authors

Hongyan Wang¹, Tianrong Xin¹, Haifeng Wang¹, Kexin Wen¹, Yimeng Liu¹, Jing Wang¹, Zhiwen Zou¹, Ling Zhong², Bin Xia¹

Affiliations

¹ School of Life Sciences, Nanchang University, Nanchang 330031, P.R.China.
² Nanchang Plant Protection and Inspection Bureau of Jiangxi Province, Nanchang 330096, P.R.China.

Abstract

The toxicity of insecticides used in the field decreases gradually to sublethal concentrations over time. Therefore, it is necessary to study sublethal effects of pesticides for controlling population explosion. Panonychus citri is a global pest which control is based on insecticides. This study explores the stress responses of spirobudiclofen on the P. citri. Spirobudiclofen significantly inhibited survival and reproduction of P. citri, and the effects aggravated as concentration increased. The transcriptomes and metabolomes of spirobudiclofen-treated and control were compared to characterize spirobudiclofen molecular mechanism. Transcriptomics indicated stress induced by spirobudiclofen stimulated immune defense, antioxidative system, cuticle formation, and lipid metabolism, as deduced from RNA-seq analysis. Meanwhile, our study found that tolerance metabolism in P. citri was regulated by promoting the metabolism of glycerophospholipids, glycine, serine, and threonine. The results of this study can provide a basis for exploring the adaptation strategies of P. citri to spirobudiclofen stress.

Keywords: Agricultural science; Entomology; Metabolomics; Transcriptomics.