Screening for IBs-relative genes by transcriptome analysis and generation IBs-less mutants in Culter alburnus

Jianbo Zheng; Changxi He; Wenping Jiang; Shili Liu; Fei Li; Meili Chi; Shun Cheng; Yinuo Liu

doi:10.1016/j.cbd.2023.101106

Screening for IBs-relative genes by transcriptome analysis and generation IBs-less mutants in Culter alburnus

Comp Biochem Physiol Part D Genomics Proteomics. 2023 Sep:47:101106. doi: 10.1016/j.cbd.2023.101106. Epub 2023 Jun 23.

Authors

Jianbo Zheng¹, Changxi He², Wenping Jiang¹, Shili Liu¹, Fei Li³, Meili Chi¹, Shun Cheng¹, Yinuo Liu¹

Affiliations

¹ Key Laboratory of Genetics and Breeding, Zhejiang Institute of Freshwater Fisheries, Huzhou, China.
² Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Engineering Research Center of Aquaculture, National Demonstration Center for Experimental Fisheries Science Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
³ Key Laboratory of Genetics and Breeding, Zhejiang Institute of Freshwater Fisheries, Huzhou, China. Electronic address: lifeibest1022@163.com.

PMID: 37413699
DOI: 10.1016/j.cbd.2023.101106

Abstract

Intermuscular bones (IBs), distributed specifically in the myosepta on both sides of lower teleosts, negatively affect palatability and processing. Recent research in zebrafish and several economically important farmed fishes has led to the breakthrough discovery of the mechanism of IBs formation and generation of IBs-loss mutants. This study explored the ossification patterns of IBs in juvenile Culter alburnus. Besides, some key genes and bone-related signaling pathways were identified by transcriptomic data. Furthermore, PCR microarray validation revealed that claudin1 could potentially regulate IBs formation. Additionally, we created several IBs-reduced mutants of C. alburnus by loss of the function of bone morphogenetic proteins 6 (bmp6) gene using CRISPR/Cas9 editing. These results suggested that CRISPR/Cas9-mediated bmp6 knockout was promising approach for breeding IBs-free strain in other cyprinids.

Keywords: CRISPR/Cas9; Culter alburnus; Intermuscular bones; bmp6; claudin1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone and Bones
Cyprinidae* / genetics
Cyprinidae* / metabolism
Gene Expression Profiling
Transcriptome
Zebrafish* / genetics