Negative Ion-Mode N-Glycan Mass Spectrometry Imaging by MALDI-2-TOF-MS

Jens Soltwisch; Bram Heijs

doi:10.1007/978-1-0716-3319-9_15

Negative Ion-Mode N-Glycan Mass Spectrometry Imaging by MALDI-2-TOF-MS

Methods Mol Biol. 2023:2688:173-186. doi: 10.1007/978-1-0716-3319-9_15.

Authors

Jens Soltwisch^{1

2}, Bram Heijs³

Affiliations

¹ Center for Proteomics & Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
² Biomedical Mass Spectrometry, Institute of Hygiene, University Hospital Munster, Münster, Germany.
³ Center for Proteomics & Metabolomics, Leiden University Medical Center, Leiden, The Netherlands. B.P.A.M.Heijs@lumc.nl.

PMID: 37410293
DOI: 10.1007/978-1-0716-3319-9_15

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry imaging with laser-induced postionization (MALDI-2-MSI) has proven a powerful tool for the in situ analysis of N-linked glycosylation, or N-glycans, directly from clinical tissue samples. Here we describe a sample preparation protocol for the analysis of N-glycans from formalin-fixed, paraffin-embedded tissue sections.

Keywords: Laser-induced postionization; MALDI-2; Mass spectrometry imaging; Matrix-assisted laser desorption/ionization; N-Linked glycosylation; Oligosaccharides; On-tissue enzymatic digestion.

MeSH terms

Diagnostic Imaging*
Glycosylation
Paraffin Embedding
Polysaccharides* / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

Polysaccharides