Spatially Resolved Quantitation of Drug in Skin Equivalents Using Mass Spectrometry Imaging (MSI)

Cristina Russo; Malcolm R Clench

doi:10.1007/978-1-0716-3319-9_3

Spatially Resolved Quantitation of Drug in Skin Equivalents Using Mass Spectrometry Imaging (MSI)

Methods Mol Biol. 2023:2688:27-40. doi: 10.1007/978-1-0716-3319-9_3.

Authors

Cristina Russo^{1

2}, Malcolm R Clench³

Affiliations

¹ Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK. C.Russo@mdx.ac.uk.
² Department of Natural Sciences, Middlesex University, London, UK. C.Russo@mdx.ac.uk.
³ Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.

PMID: 37410281
DOI: 10.1007/978-1-0716-3319-9_3

Abstract

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has seen a growing interest as a leading technique in the pharmaceutical industry for mapping label-free exogenous and endogenous species in biological tissues. However, the use of MALDI-MSI to perform spatially resolved absolute quantitation of species directly in tissues is still challenging, and robust quantitative mass spectrometry imaging (QMSI) methods need to be developed. In this study, we describe the microspotting technique for analytical and internal standard deposition, matrix sublimation, powerful QMSI software, and mass spectrometry imaging setup to obtain absolute quantitation of drug distribution in 3D skin models.

Keywords: 3D skin models; Internal standard; MALDI-MSI; Microspotting; QMSI; Quantitation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Diagnostic Imaging*
Skin*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods