Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells

Clarisse Cornebise; Maude Perus; François Hermetet; Josep Valls-Fonayet; Tristan Richard; Virginie Aires; Dominique Delmas

doi:10.3390/cells12101408

Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells

Cells. 2023 May 17;12(10):1408. doi: 10.3390/cells12101408.

Authors

Clarisse Cornebise^{1

2}, Maude Perus^{1

2}, François Hermetet^{1

2}, Josep Valls-Fonayet³, Tristan Richard³, Virginie Aires^{1

2}, Dominique Delmas^{1

2

4}

Affiliations

¹ UFR des Sciences de Santé, Université de Bourgogne, 21000 Dijon, France.
² INSERM Research Center U1231-Cancer and Adaptive Immune Response Team, Bioactive Molecules and Health Research Group, 21000 Dijon, France.
³ Université de Bordeaux, Bordeaux Sciences Agro, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, 33140 Villenave d'Ornon, France.
⁴ Centre de Lutte Contre le Cancer Georges François Leclerc Center, 21000 Dijon, France.

Abstract

Age-related macular degeneration (AMD) is one of the most commonly occurring ocular diseases worldwide. This degenerative condition affects the retina and leads to the loss of central vision. The current treatments are focused on the late stage of the disease, but recent studies have highlighted the importance and benefits of preventive treatments and how good dietary habits can reduce the risk of progression to an advanced form of the disease. In this context, we studied whether resveratrol (RSV) or a polyphenolic cocktail, red wine extract (RWE), are able to prevent the initiating events of AMD (i.e., oxidative stress and inflammation) in human ARPE-19 retinal pigment epithelial (RPE) cells and macrophages. This study highlights that RWE and RSV can prevent hydrogen peroxide (H₂O₂) or 2,2'-Azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative stress and can subsequently prevent DNA damage via the inhibition of the ATM (ataxia telangiectasia-mutated)/Chk2 (checkpoint kinase 2) or Chk1 signaling pathways, respectively. Moreover, ELISA assays show that RWE and RSV can prevent the secretion of proinflammatory cytokines in RPE cells and in human macrophages. Interestingly, RWE exhibits a greater protective impact compared to RSV alone, even though RSV was more concentrated when used alone than in the red wine extract. Our results suggest that RWE and RSV may have potential interest as preventive nutritional supplementations against AMD.

Keywords: AMD; inflammation; oxidative stress; polyphenol; prevention; red wine extract; resveratrol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Hydrogen Peroxide
Inflammation / drug therapy
Inflammation / metabolism
Macular Degeneration* / metabolism
Oxidative Stress
Resveratrol / pharmacology
Wine*

Substances

Hydrogen Peroxide
Resveratrol

Grants and funding

C.C. was supported by grants from the “Fonds Européen de Développement Régional” (FEDER) and the “Bureau interprofessionnel des Vins de Bourgogne“ (BIVB). M.P. was supported by grants from ANRT no. 2021/1248 by the French government. This work was supported by a French government grant managed by the French National Research Agency under the program “Investissements d’Avenir”, reference ANR-11-LABX-0021, and was supported by grants from the “Conseil Régional de Bourgogne”, the “Fonds Européen de Développement Régional” (FEDER), the “Bureau interprofessionnel des Vins de Bourgogne” (BIVB).