Slit dual-frequency ultrasound-assisted pulping of Lycium barbarum fresh fruit to improve the dissolution of polysaccharides and in situ real-time monitoring

Tianyu Kong; Shuhan Liu; Yuqin Feng; Yanli Fan; Junwei Yu; Haihui Zhang; Meihong Cai; Haile Ma; Yuqing Duan

doi:10.1016/j.ultsonch.2023.106509

Slit dual-frequency ultrasound-assisted pulping of Lycium barbarum fresh fruit to improve the dissolution of polysaccharides and in situ real-time monitoring

Ultrason Sonochem. 2023 Aug:98:106509. doi: 10.1016/j.ultsonch.2023.106509. Epub 2023 Jul 1.

Authors

Tianyu Kong¹, Shuhan Liu¹, Yuqin Feng¹, Yanli Fan², Junwei Yu³, Haihui Zhang⁴, Meihong Cai¹, Haile Ma⁵, Yuqing Duan⁶

Affiliations

¹ School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
² School of Food & Wine, Ningxia University, Yinchuan 750021, China.
³ Ningxia Zhongning Goji Industry Innovation Research Institute Co., Ltd, Zhongning 755100, China.
⁴ School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: zhanghh@ujs.edu.cn.
⁵ School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China.
⁶ School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China; Nourse Pet Nutrition Jiangsu Research Institute, Zhenjiang 212009, China. Electronic address: dyq101@ujs.edu.cn.

Abstract

In this study, the slit dual-frequency ultrasound-assisted pulping of fresh Lycium barbarum fruit was optimized to improve the dissolution of polysaccharides. The microscopic mechanism of polysaccharide dissolution was explored through establishing polysaccharides dissolution kinetics model and visualizing the multi-physical fields during ultrasonic process, and an in situ real-time monitoring model was established by the relationship between the chemical value and spectral information collected by near-infrared spectroscopy. The results showed that, under optimal conditions, treatment with ultrasound (28-33 kHz, 250 W, 30 min) not only significantly promoted the dissolution rate of polysaccharides in Lycium barbarum pulp (LBPPs, increased by 43.64 %, p < 0.01), reduced its molecular weight, but also improved the arabinose molar ratio, the uniformity of polysaccharide particles, and the antioxidant activity of LBPPs. Correlation analysis indicated that ultrasonic treatment is closely related to LBPPs content, particle size and scavenging capacity against superoxide anion radicals (p_{total sugar content} < 0.01, p_{particle size} < 0.05 and p_{superoxide anion scavenging} < 0.05). Moreover, the in situ real-time monitoring model for the pulping process could quantitatively predict LBPPs dissolution rate and its superoxide anion radical scavenging capacity with good calibration and prediction performance (Rc = 0.9841, RMSECV = 0.0873, Rp = 0.9772, RMSEP = 0.0530; Rc = 0.9874, RMSECV = 0.1246, Rp = 0.9868, RMSEP = 0.0665). These results indicated that slit dual-frequency ultrasound has great potential in improving the quality of Lycium barbarum pulp, which may provide theoretical support for the industrial development of intelligent systems for polysaccharides preparation.

Keywords: Antioxidant; Dissolution kinetic model; In situ real-time monitoring; Lycium barbarum pulp; Polysaccharides; Structural properties.

MeSH terms

Drugs, Chinese Herbal* / analysis
Fruit / chemistry
Lycium* / chemistry
Polysaccharides / chemistry
Solubility
Superoxides

Substances

Superoxides
Polysaccharides
Drugs, Chinese Herbal