In this paper, we designed, synthesized, and characterized 4,4',4'',4'''-(ethene-1,1,2,2-tetrayl)tetrakis(N,N-dimethylaniline) (1). Furthermore, UV-Vis absorbance and fluorescence emission studies reveal that 1 acts as a selective and sensitive probe for reversible acid-base sensing in solution as well as in the solid state. Nevertheless, the probe exhibited colorimetric sensing and intracellular fluorescent cell imaging of acid-base sensitive cells, making it a practical sensor with several potential applications in chemistry.