Single-cell RNA sequencing for the study of kidney disease

Jiayi Zhu; Jinrong Lu; Huachun Weng

doi:10.1186/s10020-023-00693-8

Single-cell RNA sequencing for the study of kidney disease

Mol Med. 2023 Jul 3;29(1):85. doi: 10.1186/s10020-023-00693-8.

Authors

Jiayi Zhu^#¹, Jinrong Lu^#¹, Huachun Weng²

Affiliations

¹ The College of Medical Technology, Shanghai University of Medicine & Health Sciences, 279 Zhouzhu Highway, Pudong New Area, 201318, Shanghai, China.
² The College of Medical Technology, Shanghai University of Medicine & Health Sciences, 279 Zhouzhu Highway, Pudong New Area, 201318, Shanghai, China. Wenghc@sumhs.edu.cn.

^# Contributed equally.

Abstract

The kidney is an important organ for maintaining normal metabolism and stabilising the internal environment, in which, the heterogeneity of cell types has hindered the progress in understanding the mechanisms underlying kidney disease. In recent years the application of single-cell RNA sequencing (scRNA-seq) in nephrology has developed rapidly. In this review, we summarized the technical platform related to scRNA-seq and the role of this technology in investigating the onset and development of kidney diseases, starting from several common kidney diseases (mainly including lupus nephritis, renal cell carcinoma, diabetic nephropathy and acute kidney injury), and provide a reference for the application of scRNA-seq in the study of kidney disease diagnosis, treatment and prognosis.

Keywords: Kidney disease; Pathogenesis; scRNA-seq.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma, Renal Cell*
Diabetic Nephropathies*
Gene Expression Profiling
Humans
Kidney
Kidney Neoplasms*
Sequence Analysis, RNA