Objective: To establish a high performance liquid chromatography method for the determination of misoprostol in workplace air. Methods: From February to August 2021, the misoprostol in the workplace air was collected by glass fiber filter membrane, and theeluent was separated by C18 liquid chromatography column, determined by UV detector, and quantified by external standard method. Results: The quantitative lower limit of misoprostol determination method was 0.05 μg/ml, and the lowest quantitative concentration was 1.4 μg/m(3) (calculated by collecting 75 L air sample). The concentration of misoprostol has a good linear relationship between 0.05 to 10.00 μg/ml. The relative coefficient was 0.9998. The regression equation of the standard working curve was y=495759x-45257. The range of average recovery rates were from 95.5% to 102.8%. The intra-assay precision of the method was 1.2%-4.6%, and the inter-assay precision was 2.0%-5.9%. The samples could be stored stably for 7 days at 4 ℃. Conclusion: The high performance liquid chromatography method for the determination of misoprostol has high sensitivity, good specificity and simple procedure of sample pretreatment. It is suitable for the detection of misoprostol in the workplace air.
目的： 建立工作场所空气中米索前列醇的高效液相色谱测定方法。 方法： 于2021年2至8月，工作场所空气中的米索前列醇用玻璃纤维滤膜采集，洗脱液经C18液相色谱柱分离，紫外检测器检测，外标法定量。 结果： 方法定量下限为0.05 μg/ml，最低定量浓度为1.4 μg/m(3)（以采集75 L空气样品计算)，米索前列醇浓度在0.05~10.00 μg/ml范围内线性关系良好，相关系数为0.999 8。本次标准工作曲线回归方程y=495 759x-45 257。方法的平均回收率为95.5%~102.8%；批内精密度为1.2%~4.6%，批间精密度为2.0%~5.9%；样品在4 ℃条件下可稳定保存7 d。 结论： 米索前列醇的高效液相色谱测定方法灵敏度高，特异性好，样品前处理步骤简单，适用于工作场所空气中米索前列醇的检测。.
Keywords: Air; Chromatography, high performance liquid; Misoprostol; Workplace.