Paper-based biosensors relying on core biological immune scaffolds for the detection of procymidone in vegetables

Gaozhen Li; Jiashuai Sun; Jiahuan Li; Yaoli Zhang; Jingcheng Huang; Fengling Yue; Haowei Dong; Falan Li; Huihui Xu; Yanyin Guo; Yemin Guo; Xia Sun

doi:10.1016/j.talanta.2023.124843

Paper-based biosensors relying on core biological immune scaffolds for the detection of procymidone in vegetables

Talanta. 2023 Dec 1:265:124843. doi: 10.1016/j.talanta.2023.124843. Epub 2023 Jun 20.

Authors

Gaozhen Li¹, Jiashuai Sun¹, Jiahuan Li², Yaoli Zhang², Jingcheng Huang¹, Fengling Yue¹, Haowei Dong¹, Falan Li¹, Huihui Xu², Yanyin Guo², Yemin Guo³, Xia Sun⁴

Affiliations

¹ School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China.
² School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China.
³ School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China. Electronic address: gym@sdut.edu.cn.
⁴ School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China. Electronic address: sunxia2151@sina.com.

PMID: 37399648
DOI: 10.1016/j.talanta.2023.124843

Abstract

In order to achieve a highly sensitive detection of procymidone in vegetables, three paper-based biosensors based on a core biological immune scaffold (CBIS) were developed, which were time-resolved fluorescence immunochromatography strips with Europium (III) oxide (Eu-TRFICS). Goat anti-mouse IgG and europium oxide time-resolved fluorescent microspheres formed secondary fluorescent probes. CBIS was formed by secondary fluorescent probes and procymidone monoclonal antibody (PCM-Ab). The first type of Eu-TRFICS (Eu-TRFICS-(1)) fixed secondary fluorescent probes on a conjugate pad, and PCM-Ab was mixed with a sample solution. The second type of Eu-TRFICS (Eu-TRFICS-(2)) fixed CBIS on the conjugate pad. The third type of Eu-TRFICS (Eu-TRFICS-(3)) was directly mixed CBIS with the sample solution. They solved the problems of steric hindrance of antibody labeling, insufficient exposure of antigen recognition region and easy loss of activity in traditional methods. They realized multi-dimensional labeling and directional coupling. They replaced the loss of antibody activity. And the three types of Eu-TRFICS were compared, among which Eu-TRFICS-(1) was the best detection choice. Antibody usage was reduced by 25% and sensitivity was increased by 3 times. Its detection range was 1-800 ng/mL, the limit of detection (LOD) was 0.12 ng/mL with the visible LOD (vLOD) of 5 ng/mL.

Keywords: Core biological immune scaffold; Paper-based biosensors; Procymidone; Time-resolved fluorescence immunochromatography strips; Vegetables.