Stimulus contrast modulates burst activity in the lateral geniculate nucleus

Alyssa N Sanchez; Henry J Alitto; Daniel L Rathbun; Tucker G Fisher; W Martin Usrey

doi:10.1016/j.crneur.2023.100096

Stimulus contrast modulates burst activity in the lateral geniculate nucleus

Curr Res Neurobiol. 2023 Jun 10:4:100096. doi: 10.1016/j.crneur.2023.100096. eCollection 2023.

Authors

Alyssa N Sanchez¹, Henry J Alitto¹, Daniel L Rathbun², Tucker G Fisher³, W Martin Usrey¹

Affiliations

¹ Center for Neuroscience, University of California Davis, 95618, USA.
² Dept. of Ophthalmology, Detroit Inst. of Ophthalmology, Henry Ford Health System, Detroit, MI, 48202, USA.
³ Department of Neurobiology, Stanford University, CA, 94305, USA.

Abstract

Burst activity is a ubiquitous feature of thalamic neurons and is well documented for visual neurons in the lateral geniculate nucleus (LGN). Although bursts are often associated with states of drowsiness, they are also known to convey visual information to cortex and are particularly effective in evoking cortical responses. The occurrence of thalamic bursts depends on (1) the inactivation gate of T-type Ca²⁺ channels (T-channels), which become de-inactivated following periods of increased membrane hyperpolarization, and (2) the opening of the T-channel activation gate, which has voltage-threshold and rate-of-change (δv/δt) requirements. Given the time/voltage relationship for the generation of Ca²⁺ potentials that underlie burst events, it is reasonable to predict that geniculate bursts are influenced by the luminance contrast of drifting grating stimuli, with the null phase of higher contrast stimuli evoking greater hyperpolarization followed by a larger dv/dt than the null phase of lower contrast stimuli. To determine the relationship between stimulus contrast and burst activity, we recorded the spiking activity of cat LGN neurons while presenting drifting sine-wave gratings that varied in luminance contrast. Results show that burst rate, reliability, and timing precision are significantly greater with higher contrast stimuli compared with lower contrast stimuli. Additional analysis from simultaneous recordings of synaptically connected retinal ganglion cells and LGN neurons further reveals the time/voltage dynamics underlying burst activity. Together, these results support the hypothesis that stimulus contrast and the biophysical properties underlying the state of T-type Ca²⁺ channels interact to influence burst activity, presumably to facilitate thalamocortical communication and stimulus detection.

Keywords: Cat; Coding; Cortex; LGN; Retina; Thalamus.