In vivo transmission electron microscopy of the alternating structure of the protrusions between adjacent macrophage-like cells on micropatterns

Natsumi Ushijima; Tsukasa Akasaka; Shinichiro Numamoto; Tsubura Kudo; Atsuro Yokoyama

doi:10.1016/j.job.2023.06.003

In vivo transmission electron microscopy of the alternating structure of the protrusions between adjacent macrophage-like cells on micropatterns

J Oral Biosci. 2023 Sep;65(3):259-263. doi: 10.1016/j.job.2023.06.003. Epub 2023 Jun 29.

Authors

Natsumi Ushijima¹, Tsukasa Akasaka², Shinichiro Numamoto³, Tsubura Kudo³, Atsuro Yokoyama³

Affiliations

¹ Support Section for Education and Research, Faculty of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan. Electronic address: ntm@den.hokudai.ac.jp.
² Department of Biomaterials and Bioengineering, Faculty of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan.
³ Oral Functional Prosthodontics, Division of Oral Functional Science, Faculty of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan.

PMID: 37393036
DOI: 10.1016/j.job.2023.06.003

Abstract

Objectives: We investigated the behavior of macrophages in the defined microtopography of materials.

Methods: Patterned cyclo-olefin polymer films were implanted into the femurs of seven-week-old rats. After 1 and 4 weeks, the rats were fixed with glutaraldehyde and OsO₄, and their bones were observed with transmission electron microscopy (TEM).

Results: TEM and segmentation revealed an alternating structure in which multiple protrusions of adjacent macrophage-like cells overlapped. They were approximately 2 μm long and almost uniform in width, and were induced by the limited topography.

Conclusion: New structures appeared between the macrophage-like cells as a result of microtopography.

Keywords: Biomaterials; Macrophage protrusions; Microtopography; TEM; Ultrastructure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Glutaral
Macrophages*
Microscopy, Electron, Transmission
Rats

Substances

Glutaral