Light-responsive benzobisthiazole as oxidase mimic for rapid determination of glutathione in food and vegetable

Jiao Wu; Lingbo Qu; Zhaohui Li; Linping Zhao; Yuanqiang Sun; Ran Yang

doi:10.1016/j.foodchem.2023.136672

Light-responsive benzobisthiazole as oxidase mimic for rapid determination of glutathione in food and vegetable

Food Chem. 2023 Nov 30:427:136672. doi: 10.1016/j.foodchem.2023.136672. Epub 2023 Jun 29.

Authors

Jiao Wu¹, Lingbo Qu², Zhaohui Li¹, Linping Zhao³, Yuanqiang Sun¹, Ran Yang⁴

Affiliations

¹ College of Chemistry, Green Catalysis Center, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Zhengzhou University, Zhengzhou 450001, China.
² College of Chemistry, Green Catalysis Center, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Zhengzhou University, Zhengzhou 450001, China; Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Market Regulation, Zhengzhou 450001, China. Electronic address: qulingbo@zzu.edu.cn.
³ Zhengzhou Zhongdao Biotechnology Company Limited, Zhengzhou, China.
⁴ College of Chemistry, Green Catalysis Center, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Zhengzhou University, Zhengzhou 450001, China; Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Market Regulation, Zhengzhou 450001, China. Electronic address: yangran@zzu.edu.cn.

PMID: 37392627
DOI: 10.1016/j.foodchem.2023.136672

Abstract

Accurate determination of glutathione (GSH) in food and vegetable is significant to instruct the appropriate supplementation of GSH in the human body. Light-responsive enzyme mimics have been widely used in detecting GSH due to controllable temporal and spatial accuracy. However, exploring a potential organic mimic enzyme with excellent catalytic efficiency keeps challenging. Herein, a benzobisthiazole organic oxidase mimic was successfully prepared by a simple and low-cost method. Based on its high light-responsive oxidase-like activity, it was used for high reliable colorimetric determination of GSH in food and vegetable for only 1 min with a large linear range of 0.02-30 μM and a low detection limit of 5.3 nM. This study provides a novel strategy to obtain powerful light-responsive oxidase mimics and holds great potential for rapid and accurate detection of GSH in food and vegetables.

Keywords: Benzobisthiazole; Colorimetric detection; Light-responsive; Oxidase-like activity; glutathione (GSH).

MeSH terms

Colorimetry / methods
Glutathione
Humans
Oxidoreductases*
Vegetables*

Substances

Oxidoreductases
Glutathione