Hydrogels, as biological materials, are widely used in food, tissue engineering, and biomedical applications. Nevertheless, many issues remain in the preparation of hydrogels by physical and chemical methods, such as low bioaffinity, weak mechanical properties, and unstable structures, which also limit their applications in other fields. However, the enzymatic cross-linking method has the advantages of high catalytic efficiency, mild reaction conditions, and the presence of nontoxic substances. In this review, we evaluated the chemical, physical, and biological methods of preparing hydrogels and introduced three common cross-linking enzymes and their principles for preparing hydrogels. This review introduced the applications and properties of hydrogels prepared by the enzymatic method and also provided some suggestions regarding the current situation and future development of hydrogels prepared by enzymatic cross-linking.
Keywords: application; cross-linking enzymes; hydrogel; performance indices; preparation methods.