Advanced imaging techniques for tracking drug dynamics at the subcellular level

Chengying Zhang; Zhiqi Tian; Rui Chen; Fiona Rowan; Kangqiang Qiu; Yujie Sun; Jun-Lin Guan; Jiajie Diao

doi:10.1016/j.addr.2023.114978

Advanced imaging techniques for tracking drug dynamics at the subcellular level

Adv Drug Deliv Rev. 2023 Aug:199:114978. doi: 10.1016/j.addr.2023.114978. Epub 2023 Jun 28.

Authors

Chengying Zhang¹, Zhiqi Tian¹, Rui Chen², Fiona Rowan¹, Kangqiang Qiu¹, Yujie Sun², Jun-Lin Guan¹, Jiajie Diao³

Affiliations

¹ Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
² Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, USA.
³ Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA. Electronic address: jiajie.diao@uc.edu.

PMID: 37385544
PMCID: PMC10527994 (available on 2024-08-01)
DOI: 10.1016/j.addr.2023.114978

Abstract

Optical microscopes are an important imaging tool that have effectively advanced the development of modern biomedicine. In recent years, super-resolution microscopy (SRM) has become one of the most popular techniques in the life sciences, especially in the field of living cell imaging. SRM has been used to solve many problems in basic biological research and has great potential in clinical application. In particular, the use of SRM to study drug delivery and kinetics at the subcellular level enables researchers to better study drugs' mechanisms of action and to assess the efficacy of their targets in vivo. The purpose of this paper is to review the recent advances in SRM and to highlight some of its applications in assessing subcellular drug dynamics.

Keywords: Absorption and transport; Distribution; Drug design; Metabolism and excretion; Nanoparticles; Super-resolution microscopy.

Publication types

Review

Grants and funding

R35 GM128837/GM/NIGMS NIH HHS/United States