Association of bovine serum albumin and cetyltrimethylammonium chloride: An investigation of the effects of temperature and hydrotropes

Sumaya Islam Progga; Javed Masood Khan; Tajmul Hasan; Md Abdul Goni; Ashraful Alam; Dileep Kumar; Shahed Rana; Md Anamul Hoque

doi:10.1016/j.ijbiomac.2023.125592

Association of bovine serum albumin and cetyltrimethylammonium chloride: An investigation of the effects of temperature and hydrotropes

Int J Biol Macromol. 2023 Aug 15:246:125592. doi: 10.1016/j.ijbiomac.2023.125592. Epub 2023 Jun 27.

Authors

Sumaya Islam Progga¹, Javed Masood Khan², Tajmul Hasan¹, Md Abdul Goni³, Ashraful Alam¹, Dileep Kumar⁴, Shahed Rana¹, Md Anamul Hoque¹

Affiliations

¹ Department of Chemistry, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
² Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
³ Department of Biological and Physical Sciences, South Carolina State University, Orangeburg, SC 29117, USA.
⁴ Laboratory for Chemical Computation and Modeling, Institute for Computational Science and Artificial Intelligence, Van Lang University, Ho Chi Minh City, Viet Nam; Faculty of Applied Technology, School of Technology, Van Lang University, Ho Chi Minh City, Viet Nam. Electronic address: kumar.dileep@vlu.edu.vn.

PMID: 37385321
DOI: 10.1016/j.ijbiomac.2023.125592

Abstract

Interactions between bovine serum albumin (BSA) and cetyltrimethylammonium chloride (CTAC) were studied utilizing conductivity approach. The critical micelle concentration (CMC), micelle ionization (α) along with counter ion binding (β) of CTAC micellization in aqueous solutions of BSA/BSA + hydrotropes (HYTs) have been computed at 298.15-323.15 K. Increase in temperatures of CTAC + BSA/BSA mixtures in HYTs resulted in elevation of CMC due to the association of chemical species in the respective systems which reduced the degree of micelle formation. CTAC + BSA consumed greater extents of surfactant species to generate micelle formation in the corresponding systems at higher temperatures. Standard free energy change associated with the assembling processes of CTAC in BSA was found negative suggesting the spontaneous nature of micellization processes. Magnitudes of ∆H_m⁰ and ∆S_m⁰ obtained from CTAC + BSA aggregation revealed the existence of H-bonding, electrostatic interactions along with hydrophobic forces among the constituents employed in the respective systems. ∆G_m⁰ The estimated thermodynamic parameters of transfer (free energy (∆G_m,tr⁰), enthalpy (∆H_m,tr⁰) and entropy (∆S_m,tr⁰)) and compensation variables (∆H_m^0,∗ and T_c) provided significant insights on the association behaviors of the CTAC + BSA system in the selected HYTs solutions.

Keywords: BSA; Hydrotropes; Thermodynamic parameters.