Three-dimensional (3D) cell culture model is a system that co-culture carriers with 3D structural materials and different types of cells in vitro to simulate the microenvironment in vivo. This novel cell culture model has been proved to be close to the natural system in vivo. In the process of cell attachment, migration, mitosis and apoptosis, it could produce biological reactions different from that of monolayer cell culture. Therefore, it can be used as an ideal model to evaluate the dynamic pharmacological effects of active substances and the metastasis process of cancer cells. This paper compared and analyzed the different characteristics of cell growth and development under two-dimensional (2D) and 3D model culture and introduced the establishment method of 3D cell model. The application progress of 3D cell culture technology in tumor model and intestinal absorption model was summarized. Finally, the application prospect of 3D cell model in the evaluation and screening of active substance was revealed. This review is expected to provide reference for the development and application of new 3D cell culture models.
三维（3D）细胞培养模型是将具有3D结构的载体与不同类型的细胞在体外共培养，以模拟体内微环境的系统。这种新型细胞培养模型经证明与体内的自然系统接近，在细胞附着、迁移、有丝分裂和凋亡等过程产生不同于单层细胞培养的生物学反应，因此可作为评价活性物质动态药理作用和癌细胞转移过程的理想模型。本文对比分析了二维（2D）和3D模型培养下细胞生长发育的不同特点，介绍了3D细胞培养模型的建立方法，重点总结了3D细胞培养技术在肿瘤模型和肠道吸收模型中的应用进展，并揭示了3D细胞培养模型在活性物质评估和筛选中的应用前景。通过本文综述，期望可为新型3D细胞培养模型的开发和应用提供参考。.
Keywords: Active substance; Application; Functional evaluation; In vitro culture; Three-dimensional cell culture.