Lactobacillus gasseri LG08 and Leuconostoc mesenteroides LM58 exert preventive effect on the development of hyperuricemia by repairing antioxidant system and intestinal flora balance

Lizhen Liang; Zihui Meng; Fei Zhang; Zhu Jianguo; Shuguang Fang; Qingang Hu; Xuna Tang; Yanan Li

doi:10.3389/fmicb.2023.1211831

Lactobacillus gasseri LG08 and Leuconostoc mesenteroides LM58 exert preventive effect on the development of hyperuricemia by repairing antioxidant system and intestinal flora balance

Front Microbiol. 2023 Jun 12:14:1211831. doi: 10.3389/fmicb.2023.1211831. eCollection 2023.

Authors

Lizhen Liang^#¹, Zihui Meng^#¹, Fei Zhang^#², Zhu Jianguo³, Shuguang Fang³, Qingang Hu², Xuna Tang², Yanan Li¹

Affiliations

¹ School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China.
² Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
³ Department of Research and Development, Wecare-Bio Probiotics Co., Ltd., Suzhou, China.

^# Contributed equally.

Abstract

Introduction: Currently, hyperuricemia has shown a surprisingly rising trend, which attracts widespread attention due to potentially major health risks. Considering the inevitable side effects of long-term medicine, probiotics are emerging as potential therapeutics due to their ability to improve uric acid metabolism and superior safety.

Methods: In our study, two strains of probiotics, Lactobacillus gasseri LG08 (LG08) and Leuconostoc mesenteroides LM58 (LM58) isolated from kimchi were evaluated for the prebiotic properties in vitro and uric-lowering effects in vivo. Here, hyperuricemia animal model and 16S rRNA gene amplicons analysis were further studied to investigate whether these probiotics exert different effects in prevention and treatment.

Results: In vivo indicators and intestinal flora immunity revealed that both LG08 and LM58 significantly prevent the development and progression of hyperuricemia, repair the antioxidant system and maintain intestinal flora balance in healthy rats, especially LM58. After hyperuricemia was formed, although the effect of LG08 and LM58 could decrease the level of uric acid, the effect to reverse and repair antioxidant levels in the body was limited.

Discussion: In our study, these findings have important implications for hyperuricemia prevention and therapy, and provided more mechanistic insights into the effect of probiotics in hyperuricemia.

Keywords: antioxidant system; hyperuricemia; prevention; probiotic; therapy.

Grants and funding

This study was funded by the National Natural Science Foundation of China under award number 82203031. This work was also funded by the Basic Research Program of Jiangsu Province and Natural Science Research of Jiangsu Higher Education Institutions of China for financial support through the award numbers BK20220375 and 22KIB350007. Additionally, this study was funded by Jiangsu Traditional Chinese Medicine Science and Technology Development under award number MS2022051 and Nanjing Medical Science and Technique Development Foundation under award number YKK21185.