Transcriptome analysis reveals that auxin promotes strigolactone-induced adventitious root growth in the hypocotyl of melon seedlings

Jingrui Li; Mi Fan; Qinqin Zhang; Guiyun Lü; Xiaolei Wu; Binbin Gong; Yubo Wang; Ying Zhang; Hongbo Gao

doi:10.3389/fpls.2023.1192340

Transcriptome analysis reveals that auxin promotes strigolactone-induced adventitious root growth in the hypocotyl of melon seedlings

Front Plant Sci. 2023 Jun 12:14:1192340. doi: 10.3389/fpls.2023.1192340. eCollection 2023.

Authors

Jingrui Li^{1

2

3}, Mi Fan^{1

2

3}, Qinqin Zhang^{1

2

3}, Guiyun Lü^{1

2

3}, Xiaolei Wu^{1

2

3}, Binbin Gong^{1

2

3}, Yubo Wang^{1

2

3}, Ying Zhang^{1

2

3}, Hongbo Gao^{1

2

3}

Affiliations

¹ College of Horticulture, Hebei Agricultural University, Baoding, China.
² Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, China.
³ Hebei Key Laboratory of Vegetable Germplasm Innovation and Utilization, Baoding, China.

Abstract

Introduction: Strigolactone (SL) and auxin are two important phytohormones involved in plant root development, but whether they show synergistic or mutual promotion effects during adventitious root (AR) formation has not been adequately explored.

Methods: In this study, we investigated the mechanisms of GR24 (synthetic SL) and indole-3-acetic acid (IAA; a type of auxin) in the formation of ARs using melon as the study material.

Results: Morphological measurements showed that the AR number, length, superficial area, and volume under the GR24 treatment were 1.60-3.27, 1.58-3.99, 2.06-3.42, and 3.00-6.11 times greater than those of the control group, respectively, at 6-10 days; the GR24+IAA treatment further promoted AR formation in melon seedlings, and the AR number, length, superficial area, and volume under the GR24+IAA treatment were 1.44-1.51, 1.28-1.73, 1.19-1.83, and 1.31-1.87 times greater than those obtained with the GR24 treatment, respectively. Transcriptome analysis revealed 2,742, 3,352, and 2,321 differentially expressed genes (DEGs) identified from the GR24 vs. control, GR24+IAA vs. control, and GR24+IAA vs. GR24 comparisons, respectively. The GR24 treatment and GR24+IAA treatment affected auxin and SL synthesis as well as components of the phytohormone signal transduction pathway, such as auxin, brassinosteroid (BR), ethylene (ETH), cytokinin (CK), gibberellin (GA), and abscisic acid (ABA). The concentrations of auxin, GA, zeatin (ZT), and ABA were evaluated using high-performance liquid chromatography (HPLC). From 6 to 10 days, the auxin, GA, and ZT contents in the GR24 treatment group were increased by 11.48%-15.34%, 11.83%-19.50%, and 22.52%-66.17%, respectively, compared to the control group, and these features were increased by 22.00%-31.20%, 21.29%-25.75%, 51.76%-98.96%, respectively, in the GR24+IAA treatment group compared with the control group. Compared to that in the control, the ABA content decreased by 10.30%-11.83% in the GR24 treatment group and decreased by 18.78%-24.00% in the GR24+IAA treatment group at 6-10 days.

Discussion: Our study revealed an interaction between strigolactone and auxin in the induction of AR formation in melon seedlings by affecting the expression of genes related to plant hormone pathways and contents.

Keywords: adventitious root; auxin; melon; phytohormone; strigolactones; transcriptome.

Grants and funding

This research was funded by the Hebei Facility Vegetables Innovation Team of Modern Agroindustry Technology (No. HBCT2021030213), the Key Research and Develop Program of Hebei (No. 22327214D), and the Hebei Agricultural Science and Technology Achievements Transformation Fund Project.