18 O enrichment of sucrose and photosynthetic and nonphotosynthetic leaf water in a C3 grass-atmospheric drivers and physiological relations

Abstract

The ¹⁸ O enrichment (Δ¹⁸ O) of leaf water affects the Δ¹⁸ O of photosynthetic products such as sucrose, generating an isotopic archive of plant function and past climate. However, uncertainty remains as to whether leaf water compartmentation between photosynthetic and nonphotosynthetic tissue affects the relationship between Δ¹⁸ O of bulk leaf water (Δ¹⁸ O_LW ) and leaf sucrose (Δ¹⁸ O_Sucrose ). We grew Lolium perenne (a C₃ grass) in mesocosm-scale, replicated experiments with daytime relative humidity (50% or 75%) and CO₂ level (200, 400 or 800 μmol mol^-1 ) as factors, and determined Δ¹⁸ O_LW , Δ¹⁸ O_Sucrose and morphophysiological leaf parameters, including transpiration (E_leaf ), stomatal conductance (g_s ) and mesophyll conductance to CO₂ (g_m ). The Δ¹⁸ O of photosynthetic medium water (Δ¹⁸ O_SSW ) was estimated from Δ¹⁸ O_Sucrose and the equilibrium fractionation between water and carbonyl groups (ε_bio ). Δ¹⁸ O_SSW was well predicted by theoretical estimates of leaf water at the evaporative site (Δ¹⁸ O_e ) with adjustments that correlated with gas exchange parameters (g_s or total conductance to CO₂ ). Isotopic mass balance and published work indicated that nonphotosynthetic tissue water was a large fraction (~0.53) of bulk leaf water. Δ¹⁸ O_LW was a poor proxy for Δ¹⁸ O_Sucrose , mainly due to opposite Δ¹⁸ O responses of nonphotosynthetic tissue water (Δ¹⁸ O_non-SSW ) relative to Δ¹⁸ O_SSW , driven by atmospheric conditions.

Keywords: 18O in leaf water and sucrose; Craig-Gordon evaporative site 18O enrichment; Lolium perenne (perennial ryegrass); atmospheric CO2 concentration; mesophyll conductance; photosynthetic and nonphotosynthetic leaf fractions; relative humidity of air; stomatal conductance; transpiration.