Proteomics Identified UDP-Glycosyltransferase Family Members as Pro-Viral Factors for Turnip Mosaic Virus Infection in Nicotiana benthamiana

Kaida Ding; Zhaoxing Jia; Penghuan Rui; Xinxin Fang; Hongying Zheng; Jianping Chen; Fei Yan; Guanwei Wu

doi:10.3390/v15061401

Proteomics Identified UDP-Glycosyltransferase Family Members as Pro-Viral Factors for Turnip Mosaic Virus Infection in Nicotiana benthamiana

Viruses. 2023 Jun 20;15(6):1401. doi: 10.3390/v15061401.

Authors

Kaida Ding^{1

2}, Zhaoxing Jia^{1

2}, Penghuan Rui^{1

2}, Xinxin Fang^{1

2}, Hongying Zheng^{1

2}, Jianping Chen^{1

2}, Fei Yan^{1

2}, Guanwei Wu^{1

2}

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
² Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.

Abstract

Viruses encounter numerous host factors that facilitate or suppress viral infection. Although some host factors manipulated by viruses were uncovered, we have limited knowledge of the pathways hijacked to promote viral replication and activate host defense responses. Turnip mosaic virus (TuMV) is one of the most prevalent viral pathogens in many regions of the world. Here, we employed an isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics approach to characterize cellular protein changes in the early stages of infection of Nicotiana benthamiana by wild type and replication-defective TuMV. A total of 225 differentially accumulated proteins (DAPs) were identified (182 increased and 43 decreased). Bioinformatics analysis showed that a few biological pathways were associated with TuMV infection. Four upregulated DAPs belonging to uridine diphosphate-glycosyltransferase (UGT) family members were validated by their mRNA expression profiles and their effects on TuMV infection. NbUGT91C1 or NbUGT74F1 knockdown impaired TuMV replication and increased reactive oxygen species production, whereas overexpression of either promoted TuMV replication. Overall, this comparative proteomics analysis delineates the cellular protein changes during early TuMV infection and provides new insights into the role of UGTs in the context of plant viral infection.

Keywords: Nicotiana benthamiana; proteomics; reactive oxygen species; turnip mosaic virus; uridine diphosphate-glycosyltransferase (UGT); viral replication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Nicotiana*
Plant Diseases
Potyvirus* / genetics
Proteomics

Supplementary concepts

Turnip mosaic virus

Grants and funding

This work was supported by grants from the National Natural Science Foundation of China (32070165) to G.W., the China Agriculture Research System of MOF and MARA (CARS-24-C-04) to F.Y., the Ningbo Major Special Projects of the Plan “Science and Technology Innovation 2025” (2021Z106) to H.Z. and the K. C. Wong Magna Fund in Ningbo University.