Grain filling plays an important role in achieving high grain yield. Manipulating planting densities is recognized as a viable approach to compensate for the reduced yield caused by nitrogen reduction. Understanding the effects of nitrogen fertilization and planting density on superior and inferior grain filling is crucial to ensure grain security. Hence, double-cropping paddy field trials were conducted to investigate the effect of three nitrogen levels (N1, conventional nitrogen application; N2, 10% nitrogen reduction; N3, 20% nitrogen reduction) and three planting densities (D1, conventional planting density; D2, 20% density increase; D3, 40% density increase) on grain yield, yield formation, and grain-filling characteristics at two sowing dates (S1, a conventional sowing date, and S2, a date postponed by ten days) in 2019-2020. The results revealed that the annual yield of S1 was 8.5-14% higher than that of S2. Reducing nitrogen from N2 to N3 decreased the annual yield by 2.8-7.6%, but increasing planting densities from D1 to D3 significantly improved yield, by 6.2-19.4%. Furthermore, N2D3 had the highest yield, which was 8.7-23.8% higher than the plants that had received the other treatments. The rice yield increase was attributed to higher numbers of panicles per m2 and spikelets per panicle on the primary branches, influenced by superior grain filling. Increasing planting density and reducing nitrogen application significantly affected grain-filling weight, with the 40% density increase significantly facilitating superior and inferior grain filling with the same nitrogen level. Increasing density can improve superior grains while reducing nitrogen will decrease superior grains. These results suggest that N2D3 is an optimal strategy to increase yield and grain filling for double-cropping rice grown under two sowing-date conditions.
Keywords: density increase; grain filling; grain yield; nitrogen reduction; sowing dates.